Striker David Trezeguet has been ruled out of Juventus' Champions League quarter-final, second-leg match at Barcelona tomorrow after failing to recover from a shoulder injury.
Trezeguet picked up the injury two weeks ago but Juve coach Marcello Lippi had been hopeful that the Frenchman would be fit to face Barcelona.
Trezeguet was not named in the travelling party due to fly to Spain later today and his place in attack will be taken by Marco Di Vaio.
Di Vaio partnered Alessandro Del Piero in the 2-1 win over AS Roma on Saturday.
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Juve face a difficult task at the Nou Camp after the first leg in Turin ended 1-1.